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The cholinergic neurons during the PPN and LDT regulate each SNc and VTA neurons by means of activating acetylcholine receptors in these neurons [8]. Combining optogenetic and brain slice patch-clamp procedures, Xiao et al. [8] demonstrated that 5–10 s of optogenetic stimulation of cholinergic projections with the PPN and LDT evokes inward curr
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